Zoë Alleyne Washburne was a female human Separatist who served in the Unification War under Count Dooku for two and a half years, and was one of the few to survive the Battle of Serenity Valley. When Malcolm and his crew betrayed the browncoats and joined the Alliance of Nations, Zoë secretly joined the CIS as a agent and later continued under his command on his spaceship, Serenity, as first mate, and was the wife of pilot Hoban Washburne. A loyal second-in-command and a tough, deadly fighter, Zoë was the only member of Mal's crew to regularly call him "sir", while taking orders as if receiving them from a superior officer.
Unknown to the crew of Serenity, she was actually a agent for the Confederacy of Independent Systems under orders of Nute Gunray and Grievous to the destruction of Serenity and the failed assassination of Padme Amidala and later was killed by Jedi Master Obi-Wan Kenobi.